Publication Abstract

Effects of steroids and human chorionic gonadotrophin on in vitro oocyte final maturation in two marine flatfish: the dab, Limanda limanda and the plaice, Pleuronectes platessa

Effects of steroids and human chorionic gonadotrophin on in vitro oocyte final maturation in two marine flatfish: the dab, Limanda limanda and the plaice, Pleuronectes platessa

A.V.M. Canario and A.P. Scott

This study investigated the role of gonadotrophin and steroids in the induction of in vitro oocyte maturation in dab (Limanda limanda) and plaice (Pleuronectes platessa), two marine flatfish (Pleuronectiformes) in which the oocytes mature and ovulate in groups (or batches). Oocytes were always more responsive to HCG than to steroids, and the median efficient doses obtained with HCG were negatively correlated with oocyte diameters. Bioassay sensitivity to steroids was low when compared with other teleosts, and intraassay variability was high, particularly in plaice. Despite the high variability, a common pattern in specificities to steroids was obtained for the two species, which was also similar to other teleost species. Among the most effective steroids found were 17alpha, 20 beta-dihydroxy-4- pregnen-3-one and 17alpha, 20beta, 12-trihydroxy-4-pregnen-3-one.

Reference:

A.V.M. Canario and A.P. Scott, 1990. Effects of steroids and human chorionic gonadotrophin on in vitro oocyte final maturation in two marine flatfish: the dab, Limanda limanda and the plaice, Pleuronectes platessa. General and Comparative Endocrinology, 77: 161-176.

A.V.M. Canario* and A.P. Scott*

General and Comparative Endocrinology, 77: 161-176