Publication Abstract

Identification of, and development of radioimmunoassys for 17alpha, 21-dihydroxy-4-pregnene-3, 20-dione (11 deooxycortisol) and 3alpha, 17 alpha, 21-trihydroxy-5beta-pregnen-20-one in the ovaries of mature male plaice, Pleuronectes platessa

Identification of, and development of radioimmunoassys for 17alpha, 21-dihydroxy-4-pregnene-3, 20-dione (11 deooxycortisol) and 3alpha, 17 alpha, 21-trihydroxy-5beta-pregnen-20-one in the ovaries of mature male plaice, Pleuronectes platessa

A.V.M. Canario and A.P. Scott

Ovaries from a female plaice (Pleuronectes platessa) that had been injected with human chorionic gonadotrophin were incubated in vitro with 17 alpha -hydroxy(1,2,6,7- ³H)progesterone. The major steroids produced by the ovaries were tentatively identified as 17alpha, 21-dihydroxy-4-pregnene-3,20di-one (11-deoxycortisol; 17,21-P), 17alpha, 21-dihydroxy-5 beta -pregnane-3,20-dione (3 alpha,17,21-P-5 beta ). Radioimmunoassays were developed for 11-deoxycortisol and for 3 alpha, 17,21-P-5 beta and were applied to fractions of mature male and female plaice plasmas and plaice ovarian incubates that had been separated on thin-layer chromatography. The presence of all three steroids, in vivo and in vitro, was confirmed. The 3alpha, 17,21-P-5 beta radioimmunoassay also identified 3alpha, 17alpha -dihydroxy-5 beta -pregnane-20-dione in all three fluids, despite the fact that this steroid was not among the radioactive incubation products of the ovary.

Reference:

A.V.M. Canario and A.P. Scott, 1990. Identification of, and development of radioimmunoassys for 17alpha, 21-dihydroxy-4-pregnene-3, 20-dione (11 deooxycortisol) and 3alpha, 17 alpha, 21-trihydroxy-5beta-pregnen-20-one in the ovaries of mature male plaice, Pleuronectes platessa. General and Comparative Endoicrinology, 78: 273-285.

A.V.M. Canario* and A.P. Scott*

General and Comparative Endoicrinology, 78: 273-285