Publication Abstract
- Title
-
Separation of rainbow trout, Salmo gairdneri, growth hormone by gel electrophoresis
- Publication Abstract
-
Separation of rainbow trout, Salmo gairdneri, growth hormone by gel electrophoresis
O. Skarphedinsson, D.M. Power and P.M. Ingleton
Pituitaries from immature (n = 12) and mature female (n = 15)ainbow trout were cultured separately in vitro and subjected to polyacrylamide gel electrophoresis. Four protein bands were identified from the immature rainbow trout and three from the adults. The material from the immature trout was used to raise antisera. Three of the bands, including those with the highest (0.74) and lowest (0.27) Rf values, produced antibodies. Immunocytochemical studies revealed that all of the antisera bound strongly to the growth hormone cells and weakly, if at all, to prolactin cells in pituitary sections from rainbow trout.
Reference:
O. Skarphedinsson, D.M. Power and P.M. Ingleton (1990) Separation of rainbow trout, Salmo gairdneri, growth hormone by gel electrophoresis. General and Comparative Endocrinology, 80: 393-398
- Publication Internet Address of the Data
- Publication Authors
-
O. Skarphedinsson*, D.M. Power* and P.M. Ingleton
- Publication Date
- January 1990
- Publication Reference
-
General and Comparative Endocrinology, 80: 393-398
- Publication DOI: https://doi.org/
