Publication Abstract
- Title
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Levels of steroids, including cortisol and 17, 20alpha-hydroxy-4-pregnen-3-one, in plasma, seminal fluid and urine of Pacific herring, Clupea herengus pallasi and North Sea plaice, Pleuronectes platessa
- Publication Abstract
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Levels of steroids, including cortisol and 17, 20alpha-hydroxy- 4-pregnen-3-one, in plasma, seminal fluid and urine of Pacific herring, Clupea herengus pallasi and North Sea plaice, Pleuronectes platessa
A.P.Scott, A.V.M. Canario, N. Sherwood and C. Warby
An recent reported that herring (Clupea harengus pallasi) milt contains high concentrations of conjugated cortisol and 17alpha, 20beta -dihydroxy-4-pregnen-3-one. The authors suggest that one source of these steroids was the urine, which could have become mixed with the milt during the hand-stripping procedure. Samples of hand-stripped milt from several other species, plaice (Pleuronectes platessa), dab (Limanda limanda), flounder (Platichthys flesus), goldfish (Carassius auratus), and rainbow trout (Oncorhynchus mykiss, formerly Salmo gairdneri), were assayed for free and conjugated cortisol. Uncontaminated samples of plasma, seminal fluid, and urine of male herring and plaice were also assayed for free and conjugated cortisol, 17alpha, 20 beta -dihydroxy-4-pregnen-3-one (herring only), 17 alpha, 20 alpha -dihydroxy-4-pregnen-3-one, and 3 alpha,17 alpha, 21-trihydroxy-5 beta -pregnan-20-one (plaice only). The results showed that hand-stripped milt from these other species, excluding rainbow trout (from which it was possible to obtain urine-free milt), also had markedly high levels of conjugated cortisol. It would appear that fish urine is a rich source of conjugated steroids and that care must be taken, when collecting milt for pheromone studies, to avoid urine contamination.
Reference:
A.P.Scott, A.V.M. Canario, N. Sherwood and C. Warby, 1991. Levels of steroids, including cortisol and 17, 20alpha-hydroxy- 4-pregnen-3-one, in plasma, seminal fluid and urine of Pacific herring, Clupea herengus pallasi and North Sea plaice, Pleuronectes platessa. Canadian Journal of Zoology, 69: 111-116.
- Publication Internet Address of the Data
- Publication Authors
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A.P.Scott*, A.V.M. Canario, N. Sherwood and C. Warby
- Publication Date
- January 1991
- Publication Reference
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Canadian Journal of Zoology, 69: 111-116
- Publication DOI: https://doi.org/
