Publication Abstract
- Title
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Rapid detection of SVC virus antigen in infected cell cultures and chemically-diseased carp by the enzyme-linked immunosorbent assay (ELISA)
- Publication Abstract
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Rapid detection of SVC virus antigen in infected cell cultures and chemically-diseased carp by the enzyme-linked immunosorbent assay (ELISA)
K. Way
An ELISA was developed using a rabbit antiserum with high levels of binding antibody against SVC virus (SVCV). Modifications were made to the standard assay which increased the sensitivity and resulted in a rapid ELISA (rELISA) which could detect SVCV antigen in cell cultures and in extracts from infected carp in approximately equals 1 hr. Virus antigen was detected by the rELISA in clinically and sub-clinically diseased carp (Cyprinus carpio) during an SVC epizootic but the sensitivity of detection of subclinical levels of virus was not as great as that of isolation of infectious virus in cell culture. However, antigen was detected in some fish extracts which failed to yield infectious virus. Fresh extracts of organs from healthy carp were found to give false positive reactions in the rELISA.
Reference:
K. Way, 1991. Rapid detection of SVC virus antigen in infected cell cultures and chemically-diseased carp by the enzyme-linked immunosorbent assay (ELISA). Journal of Applied Ichthylogy, 7: 95-107.
- Publication Internet Address of the Data
- Publication Authors
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K. Way*
- Publication Date
- January 1991
- Publication Reference
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Journal of Applied Ichthylogy, 7: 95-107
- Publication DOI: https://doi.org/
