Publication Abstract
- Title
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Use of a radioimmunoassay which detects C21 steroids with a 5b-reduced, 3a-hydroxylated configuration to to identify and measure steroids involved in final oocyte maturation in female plaice Pleuronectes platessa
- Publication Abstract
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Use of a radioimmunoassay which detects C21 steroids with a 5β-reduced, 3α-hydroxylated configuration to identify and measure steroids involved in final oocyte maturation in female plaice Pleuronectes platessa
A.P. Scott, R. Moses Inbaraj and E.L.M. Vermeirssen
Two radioimmunoassays (RIAs) have been developed which detect C21 (pregnane) steroids with a 5β-reduced, 3α-hydroxyl (5β,3α) configuration. One RIA only detects 3α,17,21-trihydroxy-5β-pregnan-20-one and 3α,17-dihydroxy-5β-pregnan-20-one, whilst the other detects a range of 5β,3α steroids, including 5P-pregnane-3α,17,20β-triol, a major metabolite of 17,20β-dihydroxy-4-pregnen-3-one, the putative oocyte maturation-inducing steroid in plaice Pleuronectes platessa. The RIAs, in conjunction with reverse-phase high-performance liquid chromatography (HPLC), have identified and quantified the steroids in plasma and urine of reproductively mature females. Total levels of 5β,3α metabolites which can be extracted with diethyl ether (i.e., free steroids) are relatively low (<10 ng/ml). However, total levels of 5β,3α metabolites released by solvolysis (i.e., sulphated steroids) are very high (up to 1000 ng/ml in plasma and 20 µg/ml in urine). On HPLC, these metabolites have been identified (in order of their abundance in plasma) as: 3α,17,21-trihydroxy-5β-pregnan-20-one, 5β-pregnane-3α,17,20(3-triol, 5β-pregnane-3α,17,20β-triol, 3α,11P,17,21-tetrahydroxy 5β-pregnane-20-one, and 3α,17-dihydroxy-5β -pregnan20-one. Levels of the first three steroids are significantly elevated in female plaice undergoing natural or gonadotrophin-induced final oocyte maturation.
Reference:
R. Moses Inbaraj, A.P. Scott and E.L.M. Vermeirssen, 1995. Use of a radioimmunoassay which detects C21 steroids with a 5b-reduced, 3a-hydroxylated configuration to identify and measure steroids involved in final oocyte maturation in female plaice Pleuronectes platessa. General and Comparative Endocrinology, 105: 50-61.
- Publication Internet Address of the Data
- Publication Authors
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R. Moses Inbaraj, A.P. Scott* and E.L.M. Vermeirssen*
- Publication Date
- January 1997
- Publication Reference
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General and Comparative Endocrinology, 105: 50-61
- Publication DOI: https://doi.org/
