Publication Abstract

Assessment of glucose oxidase behaviour in alcoholic solutions using disposable electrodes

S. Kröger, S.J. Setford and A.P.F. Turner

Solvent resistant screen-printed three-electrode devices were employed to assess the behaviour of free and immobilised glucose oxidase in water-miscible organic solvent/aqueous buffer mixtures. Three alcoholic solvents were examined, methanol, ethanol and isopropanol. A rhodinised-carbon electrocatalyst was employed to facilitate hydrogen peroxide oxidation at a decreased operating potential. The devices had a linear response range of 1-10 mU GOX and allowed detection of down to 2x 10^-14 mol GOX. The measurement of 0-1 mM glucose in 30% (v/v) ethanol was achieved in 2 min using these highly storage-stable enzyme electrodes. Such devices are likely to have widespread applications in the diagnostic and on-line monitoring fields where rapid analysis in alcoholic matrices is desirable.

Reference:

S. Kröger, S.J. Setford and A.P.F. Turner, 1998. Assessment of glucose oxidase behaviour in alcoholic solutions using disposable electrodes. Analytica Chim. Acta, 368: 219-231.