Publication Abstract
- Title
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Prolonged-release gonadotrophin releasing hormone analogue implants enhance oocyte final maturation and ovulation, and increase plasma concentrations of sulphated C21 steroids in North Sea plaice Pleuronectes platessa L
- Publication Abstract
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Prolonged-release gonadotrophinreleasing hormone analogue implants enhance oocyte final maturation and ovulation, andincrease plasma concentrations of sulphated C21 steroids in North Sea plaice Pleuronectesplatessa L
A.P. Scott, P.R. Witthames, E.L.M. Vermeirssen and J. Carolsfeld
Reproductively mature female plaice were implanted with or without50 µg of gonadotrophinreleasing hormone analogue (GnRHa), suspended in either coconut oilor methacrylate resin. The weight of the GnRHa-treated fish increased significantly (dueto hydration of the oocytes) and reached a peak between 10 and 14 days. The fish producedseveral batches of eggs, which were consistently bigger than those produced by controlfish. Plasma concentrations of free 17β-oestradiol and glucuronidated testosterone rose briefly (4 days) inresponse to the GnRHa, but then fell continuously till the end of the experiment (20days). Plasma concentrations of sulphated 5β-pregnane-3α,17,20β-triol and 5β-pregnane-3β,17,20β-triol(which are putative metabolites of 17,20β-dihydroxy-4-pregnen-3-one, the oocyte maturation-inducing steroid)increased significantly at 4 days and reached a peak between 12 and 16 days.Concentrations were still very elevated on day 20. Plasma concentrations of sulphated 3α,17,21-trihydroxy-5βpregnan -20-one showed a slightincrease on day 4 but did not change thereafter. There was a highly significant differencein the amounts of GnRHa released into the bloodstream by the two methods of administrationon day 4. However, this was not matched by significant differences in the concentrationsof any of the steroids.
Reference:
A.P. Scott, P.R. Witthames, E.L.M. Vermeirssen and J. Carolsfeld, 1999. Prolonged-releasegonadotrophin releasing hormone analogue implants enhance oocyte final maturation andovulation, and increase plasma concentrations of sulphated C21 steroids in North Seaplaice Pleuronectes platessa L. Journal of Fish Biology, 55: 316-328.
- Publication Internet Address of the Data
- Publication Authors
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A.P. Scott*, P.R. Witthames*, E.L.M. Vermeirssen* and J. Carolsfeld
- Publication Date
- January 1999
- Publication Reference
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Journal of Fish Biology, 55: 316-328
- Publication DOI: https://doi.org/
