Publication Abstract

Steroid profiles in cultured female jundi, the Siluridae Rhamdia quelen (Quoy and Gaimard, Pisces Teleostei), during the first reproductive cycle.

Steroid profiles in cultured female jundiá, the Siluridae Rhamdia quelen (Quoy and Gaimard, Pisces Teleostei), during the first reproductive cycle

Leonardo José Gil Barcellos, Viviane Mara Woehl, Alexander P. Scott, Guillermo Federico Wassermann, Rosmari Mezzalira Quevedo, István Ittzés, Irineo Fioreze and Marta Helena Krieger.

A mammalian expression vector containing the bacterial chloramphenicol acetyltransferase (CAT) gene was used to demonstrate that CAT could be successfully used as a reporter system in fish cells growing at low temperatures. We then constructed a viral haemorrhagic septicaemia virus (VHSV) minigenome by cloning the CAT reporter gene between the viral leader and trailer sequences. This construct was used in transfection experiments with helper VHSV to demonstrate that the minigenome can be encapsidated and transcribed by helper virus proteins. In addition, passaging of viruses collected from cells expressing the minigenome showed that the minigenome was being packaged and replicated in the presence of helper virus. These experiments provide the initiating steps for a reverse genetics system for VHSV.

Reference:

Leonardo José Gil Barcellos, Viviane Mara Woehl, Alexander P. Scott, Guillermo Federico Wassermann, Rosmari Mezzalira Quevedo, István Ittzés, Irineo Fioreze and Marta Helena Krieger, 2001. Steroid profiles in cultured female jundiá, the Siluridae Rhamdia quelen (Quoy and Gaimard, Pisces Teleostei), during the first reproductive cycle. General and Comparative Endocrinology, 121(3): 325-332

L.J. Barcellos, V.M. Woehl, A.P. Scott*, G.F. Wassermann, R.M. Quevedo, I. Ittzés, I. Fioreze and M. Helena Krieger.

General and Comparative Endocrinology, 121(3): 325-332