Publication Abstract
- Title
-
Comparison of biomarker and pathological responses in flounder (Platichthys flesus L.) induced by ingested polycyclic aromatic hydrocarbon (PAH) contamination.
- Publication Abstract
-
Comparison of biomarker and pathological responses in flounder (Platichthys flesus L.) induced by ingested polycyclic aromatic hydrocarbon (PAH) contamination
W.J. Reynolds, S.W. Feist, G.J. Jones, B.P. Lyons, D.A. Sheahan and G.D. Stentiford
This study investigated the response of several widely used biomarker measurements and histopathological indicators of polycyclic aromatic hydrocarbon (PAH) exposure in the flounder (Platichthys flesus L.). Flounder were fed daily with food spiked with a mixture of four PAHs at an environmentally relevant range of concentrations for either one or six months. EROD (hepatic cytochrome P450 induction) activity was elevated following 1 month exposure to PAH concentrations up to 50 mg kg-1 in food, though at higher concentrations, EROD activity was not significantly different to that of control fish. The concentration of bile metabolites (measured as 1-OH pyrene equivalents) was found to increase with PAH concentration, up to the maximum concentration tested (500 mg kg-1 PAH). By comparison, no DNA adducts (determined using the 32P-postlabelling assay) were detected and there were no significant histopathological changes in organs and tissues sampled from any of the treatment groups. Following the 6 month PAH exposure, EROD levels were not elevated, bile metabolites showed a similar dose dependant relationship as in the one month experiment, while DNA adducts were only detected in the highest PAH exposure groups. No significant histopathological changes were observed, although ultrastructurally, hepatocytes showed changes that may be associated with the effects of PAH exposure. The results are discussed with respect to the implications for the use of these methods in environmental monitoring studies.
Reference:
W.J. Reynolds, S.W. Feist, G.J. Jones, B.P. Lyons, D.A. Sheahan and G.D. Stentiford, 2003. Comparison of biomarker and pathological responses in flounder (Platichthys flesus L.) induced by ingested polycyclic aromatic hydrocarbon (PAH) contamination. Chemosphere, 52(7): 1135-1145.
- Publication Internet Address of the Data
- Publication Authors
-
W.J. Reynolds*, S.W. Feist*, G.J. Jones*, B.P. Lyons*, D.A. Sheahan* and G.D. Stentiford*
- Publication Date
- July 2003
- Publication Reference
-
Chemosphere, 52(7): 1135-1145
- Publication DOI: https://doi.org/
