Publication Abstract

Crustacean hyperglycemic hormone and hemolymph metabolites: stress responses in two lobster species

Crustacean hyperglycemic hormone and hemolymph metabolites: stress responses in two lobster species

E.S. Chang, D.M. Neil, G.D. Stentiford and S.A. Chang

Our laboratory has developed several methods for the quantification of stress in crustaceans. An ELISA was developed for the crustacean hyperglycemic hormone (CHH) from the American lobster (Homarus americanus). It is sensitive to as little as 0.2 fmol of peptide. Increases in hemolymph CHH were observed under conditions of hypoxia, elevated temperature, and altered salinity. In addition, elevated CHH concentrations were observed in Norway lobsters (Nephrops norvegicus) that were parasitized with the dinoflagellate Hematodinium and in other lobsters following the stress of capture by trawling.

Reference:

E.S. Chang, D.M. Neil, G.D. Stentiford and S.A. Chang (2005) Crustacean Hyperglycemic Hormone and Hemolymph Metabolites: Stress Responses in Two Lobster Species. In: Sakai, Y., J.P. McVey, D. Jang, E. McVey, and M. Caesar (eds.). Aquaculture and Pathobiology of Crustacean and Other Species. Proceedings of the 32nd US-Japan Meeting on Aquaculture. Davis, California, Nov. 2003. pp. 77-85

E.S. Chang, D.M. Neil, G.D. Stentiford* and S.A. Chang

In: Sakai, Y., J.P. McVey, D. Jang, E. McVey, and M. Caesar (eds.). Aquaculture and Pathobiology of Crustacean and Other Species. Proceedings of the 32nd US-Japan Meeting on Aquaculture. Davis, California, Nov. 2003. pp. 77-85