Publication Abstract

Comparison of culture media for the isolation of Renibacterium salmoninarum from naturally-infected rainbow trout (Oncorhynchus mykiss)

Comparison of culture media for the isolation of Renibacterium salmoninarum from naturally-infected rainbow trout (Oncorhynchus mykiss)

E. Chambers and G. Barker

Tissue samples from rainbow trout (Oncorhynchus mykiss) from a commercial fish farm in Southern England were tested for Renibacterium salmoninarum, using 150 fish in each of two consecutive years. Head-kidney samples from each fish were swabbed onto each of five culture media (KDM-2, KDM-C, MHC, S-KDM, and Ten-M) to compare their ability to culture R. salmoninarum from naturally-infected fish. R. salmoninarum was isolated from 80 of the 300 fish. The percentage of positives identified using each medium varied: 80% on Ten-M; 42.5% on S-KDM; 17.5% on KDM-C; 10% on KDM; and 5% on MHC. The study was extended in the second year to compare sampling locations in the kidney. Swabs from the head-kidney and anterior-kidney of the 150 fish were plated onto S-KDM. R. salmoninarum was isolated from the anterior-kidney of only 3 fish compared to 20 from the head-kidney.

Reference:

E. Chambers and G. Barker (2006) Comparison of culture media for the isolation of Renibacterium salmoninarum from naturally-infected rainbow trout (Oncorhynchus mykiss). Bulletin of the European Association of Fish Pathologists, 26(3): 137-142

E. Chambers* and G. Barker

Bulletin of the European Association of Fish Pathologists, 26(3): 137-142