Publication Abstract
- Title
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Evaluation of an alkaline phosphatase oligonucleotide probe for the detection of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus
- Publication Abstract
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Evaluation of an alkaline phosphatase oligonucleotide probe for the detection of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus
J.L. Nordstrom, R.E. Rangdale, M.C.L Vickery, A.M.B. Phillips, S.L. Murray, S. Wagley and A. DePaola
Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.
Reference
J.L. Nordstrom, R.E. Rangdale, M.C.L Vickery, A.M.B. Phillips, S.L. Murray, S. Wagley and A. DePaola (2006) Evaluation of an alkaline phosphatase oligonucleotide probe for the detection of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus. Journal of Food Protection, 69(11): 2770-2772
- Publication Internet Address of the Data
- Publication Authors
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J.L. Nordstrom, R.E. Rangdale*, M.C.L Vickery, A.M.B. Phillips, S.L. Murray, S. Wagley and A. DePaola
- Publication Date
- November 2006
- Publication Reference
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Journal of Food Protection, 69(11): 2770-2772
- Publication DOI: https://doi.org/
