Publication Abstract
- Title
-
Dual gradient LC method for the determination of pharmaceutical residues in environmental samples using a monolithic silica reversed phase column
- Publication Abstract
-
Dual gradient LC method for the determination of pharmaceutical residues in environmental samples using a monolithic silica reversed phase column
J. Bones, P. Nesterenko, K. Thomas and B. Paull
Detailed below is a simple reversed-phase liquid chromatography (RP-LC) method for the simultaneous separation of up to 21 acidic, basic, and neutral pharmaceuticals using Merck Chromolith Performance RP-C18e monolithic columns with direct ultraviolet (UV) absorption detection. By simultaneously applying a solvent elution gradient program with a mobile phase flow gradient, both a decrease in the overall analysis time and a general increase in peak efficiencies were observed. Mobile phase pH and buffer concentration were optimised using the overall resolution product under applied gradient conditions. Under optimised conditions peak area reproducibility ( n =6) ranged between 0.4 and 9.3%, determined at the method LOQ level. For real sample analysis pharmaceutical residues were extracted using an optimised solid phase extraction (SPE) procedure, utilising Strata-X extraction cartridges, which overall provided the highest relative recovery data in comparison with four other commercially available SPE sorbents (17 out of 20 residues investigated had recoveries over 70%). Complete method precision, including all sample pre-treatment and LC analysis for six spiked river water samples at the 1 and 2µgL −1 level was between 10 and 29%. Using 1L volumes of 1µgL −1 spiked estuarine water samples, the majority of detection limits were found to be in the 10–50ngL −1 range.
Reference
J. Bones, P. Nesterenko, K. Thomas and B. Paull (2006) Dual gradient LC method for the determination of pharmaceutical residues in environmental samples using a monolithic silica reversed phase column. International Journal of Environmental Analytical Chemistry, 86(7): 487-504
- Publication Internet Address of the Data
- Publication Authors
-
J. Bones, P. Nesterenko, K. Thomas* and B. Paull
- Publication Date
- November 2006
- Publication Reference
-
International Journal of Environmental Analytical Chemistry, 86(7): 487-504
- Publication DOI: https://doi.org/
