Publication Abstract

In vitro bioassay testing of produced and surface water extracts

K. V. Thomas, M. R.Hurst, W. Reynolds and J. E.Thain

Concentrated extracts of surface and produced waters were obtained using semi-permeable membrane devices (SPMDs) and large volume solid phase extraction (SPE). The activity of these extracts was then tested using a suite of in vitro bioassays. DR-CALUX was used to indicate the presence of aryl hydrocarbon receptor (AhR) agonist activity, the yeast oestrogen and androgen screen (YES & YAS) were used to test for oestrogen and androgen receptor (ER and AR) agonist activity, whilst the blue mussel (Mytilus edulis) embryo, Tisbe battagliai and Skeletonema costatum assays were used to test the toxicity of extracts to marine organisms. Data from the study are presented along with recommendations on procedures for the use of in vitro in the monitoring of the pelagic environment.

Reference:
In vitro bioassay testing of produced and surface water extracts. (2006) Biological Effects of Contaminants in Marine Pelagic Ecosystems.  K. Hylland, T. Lang & D. Vethaak (Eds.).  pp. 357-366