Publication Abstract

Phylogenetic analysis of spring viremia of carp virus reveals distinct subgroups with common origins for recent isolates in North America and the United Kingdom

Phylogenetic analysis of spring viremia of carp virus reveals distinct subgroups with common origins for recent isolates in North America and the United Kingdom

O Miller, F.J. Fuller, W.A. Gebreyes, G.A. Lewbart, I.S. Shchelkunov, R.B. Shivappa, C. Joiner, G. Woolford, D.M. Stone,  P.F. Dixon, M.E. Raley and J.F. Levine

Genetic relationships between 35 spring viremia of carp virus (SVCV) genogroup Ia isolates were determined based on the nucleotide sequence of the phosphoprotein (P) gene and glycoprotein (G) genes. Phylogenetic analysis based on P-gene sequences revealed two distinct subgroups within the SVCV genogroup Ia, designated SVCV Iai and Iaii and suggests at least two independent introductions of the virus in the US in 2002. Combined P and G-sequence data supports the emergence of SVCV in Illinois, USA and Lake Ontario, Canada from the initial outbreak in Wisconsin, and demonstrates a close genetic link to viruses isolated during routine import checks on fish imported into the UK from Asia. The data also showed a genetic link between isolations made in Missouri and Washington, USA in 2004 and the earlier isolation made in North Carolina in 2002, but based on the close relationship to a UK isolate from 2004 the data suggests that the Washington isolate represents a third introduction into the US from a common source : rather that a reemergence from the 2002 isolate. There was strong phylogenetic support for an Asian origin for 9 of 16 UK viruses either isolated from imported fish isolates or shown to have been in direct contact with fish imported from Asia, and in one case there was 100% nucleotide identity in the G-gene with a virus isolated in the Peoples Republic of China.

Reference

O Miller, F.J. Fuller, W.A. Gebreyes, G.A. Lewbart, I.S. Shchelkunov, R.B. Shivappa, C. Joiner, G. Woolford, D.M. Stone,  P.F. Dixon, M.E. Raley and J.F. Levine (2007) Phylogenetic analysis of spring viremia of carp virus reveals distinct subgroups with common origins for recent isolates in North America and the United Kingdom. Diseases of Aquatic Organisms, 76(3): 193-204

O. Miller, F.J. Fuller , W.A. Gebreyes, G.A. Lewbart, I.S. Shchelkunov, R.B. Shivappa, C. Joiner*, G. Woolford*, D.M. Stone*, P.F. Dixon*, M.E. Raley and J.F. Levine

Diseases of Aquatic Organisms, 76(3): 193-204