Publication Abstract
- Title
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A cDNA microarray for the three spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures
- Publication Abstract
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A cDNA microarray for the three spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures
F. Geoghegan, I. Katsiadaki*, T.D. Williams, and J.K. Chipman
The stickleback is a useful model species for aquatic toxicology and endocrinology and with the recent sequencing of its genome, ecotoxicogenomics. We investigated the effects on male and female stickleback gene expression of treatment with the polycyclic aromatic hydrocarbon, dibenzanthracene (DbA), the female hormone, 17-beta oestradiol (E2), and a binary mixture of the two. Real-time PCR assays were carried out for cytochrome P450 1A (CYP1A), vitellogenin and oestrogen receptor (ER) alpha and CYP1A enzyme activity was assessed by measurement of ethoxyresorufin-O-deethylase activity (EROD), vitellogenin and spiggin protein by ELISA. We developed a stickleback cDNA microarray consisting of 9692 clones and used this to assess gene expression responses to the treatments. Induction of CYP1A mRNA and EROD activity was seen in both sexes in response to DbA and a further induction of CYP1A found with the binary treatment. Vitellogenin mRNA was induced in male fish exposed to E2 and the binary mixture, while for females statistically significant induction was seen only with the binary mixture. Similar behaviour was found with zona pellucida and chorion protein mRNA using the microarray. ER-alpha mRNA was induced with E2 and the binary mixture in both sexes. The microarray showed additional transcripts that were differentially regulated by these treatments which provide novel candidates for biomarker development and mechanistic studies.
Reference
F. Geoghegan, I. Katsiadaki*, T.D. Williams, and J.K. Chipman (2008) A cDNA microarray for the three spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures. Journal of Fish Biology 72(9):2133-2153
- Publication Internet Address of the Data
- Publication Authors
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F. Geoghegan, I. Katsiadaki*, T.D. Williams, and J.K. Chipman
- Publication Date
- June 2008
- Publication Reference
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Journal of Fish Biology 72(9):2133-2153
- Publication DOI: https://doi.org/
