Publication Abstract
- Title
-
Sterol content analysis suggests altered eburicol 14a-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides
- Publication Abstract
-
Sterol content analysis suggests altered eburicol 14a-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted
T. P. Bean*, H. J. Cools, J. A. Lucas, N. D. Hawkins, J. L. Ward, M. W. Shaw and B. A. Fraaije
The recent decline in the effectiveness of some azole fungicides in controlling the wheat pathogen Mycosphaerella graminicola has been associated with mutations in the CYP51 gene encoding the azole target, the eburicol 14a-demethylase (CYP51), an essential enzyme of the ergosterol biosynthesis pathway. In this study, analysis of the sterol content of M. graminicola isolates carrying different variants of the CYP51 gene has revealed quantitative differences in sterol intermediates, particularly the CYP51 substrate eburicol. Together with CYP51 gene expression studies, these data suggest that mutations in the CYP51 gene impact on the activity of the CYP51 protein.
Reference:
T. P. Bean*, H. J. Cools, J. A. Lucas, N. D. Hawkins, J. L. Ward, M. W. Shaw and B. A. Fraaije (2009) Sterol content analysis suggests altered eburicol 14a-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted. FEMS lett 296; 266-273
- Publication Internet Address of the Data
- Publication Authors
-
T. P. Bean*, H. J. Cools, J. A. Lucas, N. D. Hawkins, J. L. Ward, M. W. Shaw and B. A. Fraaije
- Publication Date
- May 2009
- Publication Reference
-
FEMS lett 296; 266-273
- Publication DOI: https://doi.org/
